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IIS 7.5 (Windows 7) - HTTP Error 401.3 - Unauthorized | CodeAsp.Net

IIS 7.5 (Windows 7) - HTTP Error 401.3 - Unauthorized

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Today on IIS I created a virtual directory and after running it on broswer I was getting the following error:

HTTP Error 401.3 - Unauthorized
You do not have permission to view this directory or page because of the access control list (ACL) configuration or encryption settings for this resource on the Web server.

I was already having Read/Write permission on IIS_USRS:

Both IUSR and IIS_IUSRS should have access of read/write on the application folder. Below are the steps to show how to add it:

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